Formylhydrate dehydrogenase: assay and properties.

The dye reagent is buffered by M/10 phosphate salts at pH 7.4 and is prepared by dissolving 12.07 gm. anhydrous disodium hydrogen phosphate powder, 2.04 gm. potassium dihydrogen phosphate crystals, 2.60 gm. sodium arsenite powder, and 2.10 gm. sodium fluoride powder in 1 L. of distilled water. Sufficient 2,6-dichlorobenzenoneindophenol sodium is added to bring the absorbance of the solution to 0.700 at 600 m i using a 1 cm. light path and setting zero absorbance with distilled water.